Cell Biochemistry and Genomics UNIT

Ruben Fernandes, PhD

Group Leader

Ruben Fernandes, PhD
Ruben Fernandes, PhD

Full Professor - UFP

Research Topics

1. Cell physiology

We use several technologies for understanding cell viability, metabolic activity, cell proliferation, cell invasion and migration, cell death, apoptosis, senescence, among others;

2. Cell biochemistry

We study central metabolic pathways and cell signaling cascades, and organelle dynamics, using both gene expression, western-blotting, and cytochemistry stainings.

3. Computational metabolic analysis

By mens of computer tools such as COPASI, BRENDA, REACTOME, KEGG and others, we modulate metabolic pathways for drug predictive mathematic modultation.

4. Mitochondria and oxidative stress

Oxidative stress results on the imbalance of redox biochemistry. We analise glutathione reduced (GSH) and oxidized forms (GSSG), as well as the antioxidant enzymes that use them as co-factors such as glutathione peroxidase, glutathione reductase, was well as catalase, SOD, and lipid peroxidation by  thiobarbituric acid (TBA) test and its product malondialdehyde (MDA). Also by means of flow cytometry reative oxigen species are measured using fluorescent dyes DCFDA / H2DCFDA.

5. Genotoxicity and Molecular cytogenetics

By means of fluorescence in situ hybridization (FISH) we may analyze aneuploidies and microdelections. FISH is also used micronuclei genotoxicity induced by radiation. Our panel of genotoxicity assays include Ames Test in engineered E.coli, evaluation, endocrine dysruptor analysis in engineered yeasts, and also nuclei dysruption by means of single cell gel electroforesis (SCGE) or comet assay.

6. Engineered cell sensors

Our research team is developing real-time biosensors for glucose and lactate using Förster resonant energy transfer (FRET) protein interations.

7. Analytic cytometry

We use cytometry for determining DNA content, cell viability, mitocondrial membrane potential, mutliparametric analysis using specific antibodies, cell death and organelle dynamics.

Team

Carla Luis
Carla Luis

Lab Manager

Metabolism - FMUP

Adré Sousa
Adré Sousa

PhD Student

Metabolism - FMUP

Catarina Teixeira
Catarina Teixeira

MSc Student

Health Biochemistry - Clinical and Metabolic Biochemistry

Sílvia Soares
Sílvia Soares

PhD Student

Nanomedicine - UVIGO & Biomedicine - ICBAS

Ana Catarina Rocha
Ana Catarina Rocha

PhD Student

Metabolism - FMUP

Cátia Almeida
Cátia Almeida

PhD Student

Metabolism - FMUP

Patrick Pais
Patrick Pais

PhD Student

Chemical & Biological Sciences - UTAD

Diogo Ferreia
Diogo Ferreia

PhD Student

Biotechnology - UVIGO

Associated Facilities

Virology Lab

Chemical Biopathology Lab

Molecular Biology Lab

Cell culture Lab

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